Lab+7+Data+-+Section+103

In the table below, identify the loaded contents your group's wells. Your instructor will insert the gel image for your report. Be aware that anyone has the ability to edit this page so be careful not to delete data that has already been entered.

***Important Information regarding GEL image*** **LANE 6 has one band that is small did not show up. You should be able to determine it by knowing the total size of the plasmid and the size of the three bands that did show up.**

**LANE 9 has a small fragment that did not show up. Also, the large band in Lane 9 is two separate bands that did not resolve (the difference in size is ~75 bp).**

I suggest everyone use the first gel for their report.


 * Groups 1, 2, 3, and 4: 7 uL of the plasmid (donated by section 104 groups 7 and 5) was used in each lane. Gel was run for 105 min at 90 V**
 * Lane Number || Contents || Optional Comment ||
 * 1 || Ladder ||  ||
 * 2 || EcoRI ||  ||
 * 3 || HinCII ||  ||
 * 4 || EcoRI + HinCII ||  ||
 * 5 || RsaI ||  ||
 * 6 || RSAI + EcoR1 ||  ||
 * 7 || AvaI + HinCII ||  ||
 * 8 || RsaI + AvaI ||  ||
 * 9 || RsaI + HinCII ||  ||
 * 10 || EccoRI + EvaI ||  ||

Gel image(Right-click to download full-size file): This digest was incomplete. This means that you should disregard any uncut full length plasmid.
 * Groups 5, 6, 7, and 8: 4 uL of maxiprep Plasmid was loaded in each lane. The gel was run at 90 V for 113 min.**
 * Lane Number || Contents || Optional Comment ||
 * 1 || Ladder ||  ||
 * 2 || EcoRI ||  ||
 * 3 || HinCII ||  ||
 * 4 || EcoRI + HinCII ||  ||
 * 5 || RsaI ||  ||
 * 6 || RSAI + EcoR1 ||  ||
 * 7 || AvaI + HinCII ||  ||
 * 8 || RsaI + AvaI ||  ||
 * 9 || RsaI + HinCII ||  ||
 * 10 || EccoRI + EvaI ||  ||